Principle of APT Test for BacteriaĪPI Test for bacteria (API 20E Test kit) uses to identify and differentiate bacteria of family Enterobacteriaceae on the basis of biochemical tests. But here we concern with the API 20E test kit. API strips give accurate identifications based on extensive databases and are standardized, easy-to-use test systems. Modified API test kits are also available for the identification of microorganisms covering Gram-positive and Gram-negative bacteria and yeast. It is an API 20E Test kit that is quick, safe, and easy to perform and it is hence a well-established method for manual microorganism identification to the species level. ❷ Combine the bottom and lid of an incubation box and distribute water in the cells to create a humid atmosphere.API test for bacteria as shown above picture is a 20 jumbo tests kit for a biochemical panel for identification and differentiation of members of the family Enterobacteriaceae. ❶ Take a single isolated colony (from a pure culture) and make a bacterial suspension in sterile distilled water (0.5 McFarland). During incubation, bacterial metabolism produces color changes that are either spontaneous or revealed by the addition of reagents. □ A bacterial suspension is used to rehydrate each of the wells and the strips are incubated. 1- ONPG test : The ONPG test is used to detect the enzyme β-galactosidaseĢ- ADH : Decarboxylation of the amino acid arginine by arginine dihydrolaseģ- LDC: Decarboxylation of the amino acid lysine by lysine decarboxylaseĤ- ODC: Decarboxylation of the amino acid ornithine by ornithine decarboxylaseĥ- CIT : Use of citrate as sole carbon sourceĨ- TDA (Tryptophan deaminase) : Detection of the tryptophan deaminase enzyme (detected by adding Ferric chloride).ĩ- IND : Indole test : production of indole from tryptophan by the enzyme tryptophanase (Indole is detected by adding Kovac's reagent).ġ0- VP : The Voges-Proskauer test for the detection of acetoin (acetylmethylcarbinol) produced by fermentation of glucose by bacteria using the butylene glycol pathwayġ1- GEL : Production test of the enzyme gelatinase which liquefies gelatinġ2- GLU : Glucose fermentation (hexose sugar)ġ3- MAN : Fermentation of mannose (sugar hexose)ġ4- INO : Fermentation of inositol (cyclic polyalcohol)ġ5- SOR : Fermentation of sorbitol (sugar alcohol)ġ6- RHA : Rhamnose fermentation (methyl pentose sugar)ġ7- SAC : Fermentation of sucrose (disaccharide)ġ8- MEL : Fermentation of melibiose (disaccharide)ġ9- AMY : Fermentation of amygdalin (glycoside)Ģ0- ARA Fermentation of arabinose (pentose sugar)
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